This chemical is primarily utilized in bioconjugation and protein modification applications. It serves as a heterobifunctional crosslinker, enabling the attachment of biomolecules, such as proteins or peptides, to other molecules or surfaces. Its structure includes an NHS ester group, which reacts with primary amines, and a maleimide group, which selectively reacts with thiols, making it ideal for linking amine-containing and thiol-containing compounds. This functionality is particularly valuable in antibody-drug conjugates (ADCs), where it helps in creating stable and targeted drug delivery systems. Additionally, it is used in the development of diagnostic assays, immobilization of proteins on solid supports, and the study of protein-protein interactions. Its polyethylene glycol (PEG) spacer enhances solubility and reduces steric hindrance, improving the efficiency of conjugation processes.