Used primarily as a crosslinking agent in bioconjugation, this compound enables the attachment of biomolecules such as peptides, proteins, and antibodies through its maleimide and alkyne functional groups. The maleimide group selectively reacts with thiol groups (cysteine residues) under mild conditions, while the alkyne group allows for further modification via click chemistry, such as copper-catalyzed azide-alkyne cycloaddition (CuAAC). This dual reactivity makes it valuable in the development of antibody-drug conjugates (ADCs), targeted therapeutics, and functionalized biomaterials. It is also employed in proteomics research for labeling and immobilizing proteins, and in the synthesis of biosensors where controlled orientation and covalent attachment are required. Its stability in aqueous solutions and specificity in coupling reactions enhance its utility in complex biological environments.