Used as a bifunctional crosslinking agent in bioconjugation, particularly for coupling proteins and peptides. The compound contains both NHS ester and maleimide-like functionalities, enabling efficient linkage between primary amines on biomolecules and thiol groups on cysteine residues. Commonly applied in the development of antibody-drug conjugates (ADCs) and labeled proteins for diagnostic assays. Its sulfonic acid group enhances water solubility, making it suitable for reactions in aqueous buffers without organic solvents. Also employed in surface modification of biosensors and functionalization of nanoparticles for targeted delivery systems.