Used primarily as a stable isotope-labeled internal standard in analytical chemistry, particularly in mass spectrometry-based assays. It enables accurate quantification of glufosinate and related metabolites in environmental, plant, and biological samples by compensating for matrix effects and instrument variability. Its deuterated form ensures distinct mass differentiation from non-labeled compounds, enhancing detection specificity. Commonly applied in residue analysis, metabolic studies, and environmental monitoring of herbicide exposure.