GANT 58 is primarily used in biochemical research to study the Hedgehog signaling pathway, which plays a critical role in cell differentiation, proliferation, and tissue patterning during embryonic development. It acts as an inhibitor of the Gli family of transcription factors, specifically targeting Gli1 and Gli2, which are downstream effectors of the Hedgehog pathway. This makes it a valuable tool for investigating the role of Hedgehog signaling in various biological processes and diseases, including cancer. Researchers utilize GANT 58 to explore potential therapeutic strategies for cancers where the Hedgehog pathway is dysregulated, such as basal cell carcinoma and medulloblastoma. Additionally, it aids in understanding the molecular mechanisms underlying developmental disorders and tissue regeneration. Its application extends to in vitro and in vivo studies, providing insights into pathway inhibition and its effects on cellular behavior.