SANT-1 is primarily utilized in research settings to study the Hedgehog signaling pathway, which plays a critical role in cell differentiation, proliferation, and tissue patterning. It acts as a potent antagonist of the Smoothened receptor, effectively inhibiting the pathway. This property makes it a valuable tool for investigating the role of Hedgehog signaling in various biological processes, including embryonic development and cancer progression. Researchers use SANT-1 to explore potential therapeutic strategies for diseases where the Hedgehog pathway is dysregulated, such as certain types of cancers like basal cell carcinoma and medulloblastoma. Additionally, it aids in understanding the molecular mechanisms underlying these conditions, contributing to the development of targeted treatments.