HhAntag is primarily used in research settings to study the Hedgehog (Hh) signaling pathway, which plays a critical role in cell growth, tissue development, and regeneration. By inhibiting this pathway, HhAntag helps scientists understand its function in various biological processes and its involvement in diseases such as cancer. It is particularly valuable in preclinical studies to explore potential therapeutic strategies for cancers driven by abnormal Hedgehog signaling, such as basal cell carcinoma and medulloblastoma. Additionally, HhAntag is utilized in developmental biology to investigate the role of the Hedgehog pathway in embryonic development and tissue patterning. Its application extends to drug discovery, where it serves as a tool to validate targets and assess the efficacy of new compounds targeting this pathway.