SIRT1-IN-1 is primarily used as a selective inhibitor of SIRT1, a member of the sirtuin family of NAD⁺-dependent deacetylases. It is widely employed in biochemical and cellular research to study the role of SIRT1 in various signaling pathways. Applications include investigating processes related to aging, metabolism, DNA repair, and apoptosis. By inhibiting SIRT1 activity, researchers use this compound to probe the enzyme’s involvement in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. It is also utilized in mechanistic studies to validate SIRT1-targeted therapies and assess downstream effects on protein acetylation and gene expression.