LDN-57444 is primarily used in research settings as a selective inhibitor of histone deacetylase 3 (HDAC3). It has been investigated for its potential in studying cancer cell proliferation and apoptosis, as HDAC3 plays a key role in gene expression related to tumor growth. The compound is also utilized in neuroscience research to explore epigenetic mechanisms involved in neurodegenerative diseases. Due to its ability to cross the blood-brain barrier, it offers utility in studies targeting central nervous system disorders. Additionally, LDN-57444 helps in understanding immune regulation and inflammatory responses through modulation of acetylation-dependent signaling pathways. Its application remains confined to preclinical research, mainly in cellular and animal models.