HDAC-IN-3 is primarily used in research settings to study the role of histone deacetylases (HDACs) in cellular processes. It acts as an inhibitor of HDAC enzymes, which are involved in the regulation of gene expression by modifying chromatin structure. This makes it a valuable tool for investigating epigenetic mechanisms and their impact on diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. Researchers utilize HDAC-IN-3 to explore potential therapeutic strategies targeting HDAC activity, particularly in the development of treatments for diseases where abnormal gene expression plays a critical role. Its application extends to preclinical studies aimed at understanding the biological effects of HDAC inhibition and identifying novel drug candidates.