HDAC-IN-2 is primarily utilized in research focused on understanding the role of histone deacetylases (HDACs) in cellular processes. It serves as a potent inhibitor of HDAC enzymes, which are critical in regulating gene expression by modifying chromatin structure. This compound is particularly valuable in studies investigating epigenetic mechanisms and their implications in diseases such as cancer, neurodegenerative disorders, and inflammatory conditions. Researchers employ HDAC-IN-2 to explore its potential therapeutic effects, including its ability to induce cell cycle arrest, promote apoptosis, and modulate immune responses. Its application extends to preclinical models where it aids in evaluating the efficacy of HDAC inhibition as a strategy for targeted therapy. Additionally, HDAC-IN-2 is used to develop and refine novel HDAC inhibitors with improved selectivity and reduced side effects.