Dimethyl 2,5-dibromohexanedioate is primarily used as an intermediate in organic synthesis, particularly in the production of pharmaceuticals and fine chemicals. Its bromine atoms make it a valuable reagent for introducing bromo-functional groups into more complex molecules, which is essential in creating compounds with specific biological activities. Additionally, it can be employed in the development of polymers and materials, where its structure allows for controlled modifications to enhance properties like durability or reactivity. In research, it serves as a building block for synthesizing novel compounds, aiding in the exploration of new chemical reactions and pathways.