2-chloro palmitic acid is primarily used in biochemical research as a tool to study lipid metabolism and enzyme activity. It serves as a substrate or inhibitor in experiments exploring the function of enzymes like fatty acid synthase or desaturases. This compound is also utilized in the synthesis of modified lipids for investigating membrane properties and lipid-protein interactions. Additionally, it has applications in the development of surfactants and emulsifiers due to its amphiphilic nature. In industrial settings, it can be employed in the production of specialized chemicals, such as lubricants or coatings, where modified fatty acids are required.