Guanidine carbonate is widely used in the pharmaceutical industry as a raw material for synthesizing various drugs, particularly those targeting viral infections and metabolic disorders. It serves as a key intermediate in the production of antiviral medications, including those used to treat HIV and hepatitis B.

In the field of biochemistry, it is employed as a protein denaturant, helping to study protein folding and stability by disrupting hydrogen bonds. This property makes it valuable in research and diagnostic applications.

Additionally, it finds use in the production of organic compounds, such as resins, plastics, and synthetic fibers, where it acts as a catalyst or stabilizer. Its ability to enhance the physical properties of these materials makes it useful in industrial manufacturing processes.

In agriculture, guanidine carbonate is utilized as a fertilizer additive to improve nitrogen content in soil, promoting plant growth and crop yield. It is also employed as a starting material in the synthesis of pesticides.

In the cosmetics industry, it is used as an ingredient in skin care products to aid in cell exfoliation.

In the leather industry, it is used in tanning processes to condition hides, making the leather softer and more flexible.

Its versatility across multiple industries highlights its importance in both scientific and industrial applications.