1-Methylguanidine sulfate is primarily used in biochemical research as a tool to study the effects of methylated guanidine compounds on biological systems. It is often employed in experiments to investigate the role of guanidine derivatives in cellular processes, particularly in the context of urea cycle disorders and nitrogen metabolism. Additionally, it serves as a reagent in the synthesis of more complex organic compounds, aiding in the development of pharmaceuticals and other bioactive molecules. Its application extends to the study of enzyme inhibition, where it helps elucidate the mechanisms of action of various enzymes involved in metabolic pathways.