EHop-016 is primarily used in research settings for its ability to inhibit Rac1 activation, a key protein involved in cell migration and invasion. This makes it a valuable tool in cancer studies, particularly in investigating metastasis in breast cancer and other aggressive tumor types. By blocking Rac1 signaling, EHop-016 helps reduce cancer cell motility and invasiveness in vitro, offering insights into potential therapeutic strategies. It is also used to study the role of Rho GTPases in cellular processes such as cytoskeletal reorganization and membrane ruffling. Due to its specific mechanism, EHop-016 supports the development of targeted treatments aimed at preventing cancer spread rather than just tumor growth.