Used primarily in biochemical research to study enzyme activity, particularly those involved in detoxification processes like glutathione S-transferases. It serves as a substrate to investigate the metabolic pathways and mechanisms of xenobiotic compounds. Additionally, it is employed in the development of assays to measure the activity of enzymes that catalyze the conjugation of glutathione with electrophilic substances. This compound is also utilized in toxicology studies to understand the metabolism and excretion of nitroaromatic compounds. Its applications extend to the synthesis of more complex molecules for pharmaceutical research.