1-Azakenpaullone is primarily utilized in biochemical research as a potent and selective inhibitor of glycogen synthase kinase-3 (GSK-3). It plays a significant role in studying cellular signaling pathways, particularly those involved in metabolism, cell proliferation, and differentiation. Researchers employ this compound to investigate the effects of GSK-3 inhibition in various disease models, including neurodegenerative disorders like Alzheimer’s disease, where GSK-3 activity is often dysregulated. Additionally, it is used in cancer research to explore its potential in suppressing tumor growth by modulating Wnt signaling pathways. Its application extends to developmental biology studies, where it helps in understanding the role of GSK-3 in embryonic development and tissue regeneration. Overall, 1-Azakenpaullone serves as a valuable tool for probing the biological functions of GSK-3 and its implications in health and disease.