PF-03463275 is primarily used in research settings as a potent and selective inhibitor of the enzyme glycogen synthase kinase-3 (GSK-3). Its application is significant in studying the role of GSK-3 in various biological processes, including cell signaling, metabolism, and neuronal function. Researchers utilize this compound to explore its potential therapeutic effects in diseases where GSK-3 is implicated, such as Alzheimer's disease, diabetes, and mood disorders. By inhibiting GSK-3, PF-03463275 helps in understanding the pathways involved in these conditions and aids in the development of novel treatment strategies. Additionally, it is used in preclinical studies to assess its efficacy and safety profile, contributing to the broader field of drug discovery and development.