Used as a decolorizing agent in histology and microbiology, particularly in the preparation of samples for staining procedures. It helps remove excess dyes from tissue sections, improving the clarity and contrast of microscopic images. Commonly applied in the differentiation step of the Gram staining method, where it decolorizes non-acid-fast bacteria while retaining stain in acid-fast organisms. The powder form allows for easy preparation of working solutions and ensures consistent performance in laboratory settings. Also utilized in some types of fungal staining protocols to enhance specificity. Its formulation with sodium sulfite stabilizes the solution and prevents oxidation, maintaining effectiveness over time.