OGT-IN-2 is primarily used in biochemical research to study the role of O-GlcNAc transferase (OGT) in cellular processes. It serves as a potent and selective inhibitor of OGT, enabling scientists to investigate the enzyme's involvement in signaling pathways, gene expression, and protein function. This compound is particularly valuable in understanding the impact of O-GlcNAcylation on diseases such as cancer, diabetes, and neurodegenerative disorders. By modulating OGT activity, researchers can explore potential therapeutic targets and develop strategies for disease intervention. Its application extends to studying metabolic regulation and cellular stress responses, contributing to advancements in molecular biology and drug discovery.