Mutanolysin is widely used in biochemical research for its ability to break down bacterial cell walls, particularly those of Gram-positive bacteria. It is especially valuable in the study of peptidoglycan structure and function, as it cleaves specific bonds within the peptidoglycan layer. This enzyme is also employed in the preparation of protoplasts, which are cells stripped of their cell walls, enabling research on cellular processes without the barrier of the wall. Additionally, mutanolysin is utilized in the extraction of intracellular components from bacteria, facilitating the study of bacterial proteins, DNA, and other molecules. Its application extends to the development of antimicrobial strategies, as understanding cell wall degradation can inform the design of new antibiotics.