β-Glucuronidase is widely used in biochemical research and clinical diagnostics for its ability to hydrolyze glucuronide conjugates. In clinical settings, it is employed to detect and quantify glucuronidated metabolites in urine and blood samples, aiding in the diagnosis of various diseases and drug metabolism studies. In molecular biology, it serves as a reporter enzyme in gene expression assays, where its activity is measured to assess promoter strength or gene regulation. Additionally, it is utilized in the food industry to enhance the bioavailability of certain nutrients by breaking down glucuronide bonds in plant-based materials. In environmental science, β-Glucuronidase is used as an indicator of fecal contamination in water samples, helping to monitor water quality and public health safety.