β-Glucuronidase is widely used in biochemical research and medical diagnostics for its ability to hydrolyze glucuronide conjugates. In clinical settings, it is employed to detect and quantify glucuronidated metabolites in urine and blood samples, aiding in the diagnosis of certain diseases and monitoring drug metabolism. In biotechnology, it is utilized to cleave glucuronide moieties from compounds, enabling the study of bioactive molecules. Additionally, β-glucuronidase plays a role in environmental testing, where it helps assess the presence of specific pollutants by breaking down their glucuronide forms. Its application in food science includes the analysis of glucuronide-containing compounds in plant extracts and fermented products.