β-Glucosidase is widely used in the biotechnology and food industries for its ability to break down cellulose into glucose. In the production of biofuels, it plays a crucial role in converting biomass into fermentable sugars, which are then processed into ethanol. In the food industry, it is utilized to enhance the flavor and aroma of wines, teas, and fruit juices by releasing aromatic compounds from glycosides. Additionally, β-Glucosidase is employed in the pharmaceutical sector to activate certain prodrugs, improving their therapeutic efficacy. Its application in agriculture includes improving the digestibility of animal feed by breaking down complex carbohydrates into simpler sugars.