β-Mannosidase from Helix pomatia is widely used in biochemical research and industrial applications. In research, it is employed to study the structure and function of glycoproteins and glycolipids by selectively cleaving β-mannosidic linkages. This enzyme is particularly useful in glycobiology for analyzing and modifying complex carbohydrates. In the food industry, it aids in the processing of plant-based materials by breaking down mannans, improving texture and digestibility. Additionally, it finds use in the production of biofuels, where it helps degrade mannan-containing biomass into fermentable sugars. Its specificity and efficiency make it a valuable tool in both scientific and industrial settings.