This chemical is widely used in biochemical research as a fluorogenic substrate for the detection and quantification of enzyme activity, particularly for enzymes like β-N-acetylglucosaminidase. It is employed in assays to study enzyme kinetics and to measure enzyme levels in various biological samples, including cells, tissues, and body fluids. The compound releases a fluorescent product upon enzymatic hydrolysis, allowing for sensitive and specific detection using fluorescence spectroscopy or microscopy. It is also utilized in diagnostic applications to identify certain genetic disorders and metabolic diseases related to enzyme deficiencies. Additionally, it serves as a tool in microbiology to study bacterial and fungal enzymatic pathways.