Used as a chromogenic substrate in enzymatic assays to measure the activity of alpha-amylase enzymes, often in a coupled reaction with alpha-glucosidase for detection. Helps in studying carbohydrate metabolism, starch digestion, and enzyme kinetics. Applied in medical diagnostics to detect enzyme deficiencies or abnormalities, such as in pancreatic disorders. Also utilized in research to evaluate inhibitors for potential therapeutic applications in conditions like diabetes and digestive diseases. Its breakdown products, including p-nitrophenol, are easily detectable spectrophotometrically, making it a reliable tool for quantitative analysis.