Used as a fluorescent probe in biochemical and cellular studies to detect and measure enzyme activity, particularly β-glucuronidase. It is applied in research to monitor gene expression and track cellular processes due to its ability to release fluorescent 7-hydroxycoumarin upon enzymatic cleavage. Also utilized in drug metabolism studies to analyze glucuronidation pathways and assess the efficiency of drug detoxification. Additionally, it serves as a substrate in diagnostic assays for detecting bacterial infections, as certain bacteria produce β-glucuronidase. Its water-soluble nature and stable fluorescence make it a valuable tool in biomedical research and diagnostics.