Chitotetraose tetrahydrochloride hydrate is widely used in biochemical research, particularly in studies involving chitinase enzymes. It serves as a substrate to investigate the activity and specificity of these enzymes, which are crucial in understanding their role in biological processes. Additionally, it is utilized in the development of antifungal and antibacterial agents, as it mimics the natural substrates of pathogenic organisms. In the field of immunology, it is employed to study immune responses, particularly in relation to chitin recognition by the immune system. Its applications also extend to agriculture, where it is used to enhance plant defense mechanisms against fungal infections. Furthermore, it plays a role in the development of biomaterials, particularly in creating biocompatible and biodegradable products.