Resorufin-β-D-glucuronide sodium salt is widely used as a substrate in enzymatic assays, particularly for detecting β-glucuronidase activity. This enzyme is a key biomarker in various biological and pathological processes, making the compound valuable in research and diagnostics. In microbiology, it helps identify bacterial strains that produce β-glucuronidase, such as E. coli, aiding in microbial detection and differentiation. In cancer research, it is utilized to study tumor-associated β-glucuronidase activity, providing insights into cancer progression and therapeutic targeting. Additionally, it is employed in drug development to screen for inhibitors of β-glucuronidase, which can have therapeutic applications in conditions like inflammation and cancer. Its fluorescent properties make it a useful tool in high-throughput screening and imaging studies.