This chemical is widely used in biochemical research as a substrate for studying the activity of glycosidases, particularly enzymes like β-galactosidase and α-N-acetylgalactosaminidase. Its structure, which includes a p-nitrophenyl (pNP) group, allows for easy detection of enzymatic activity through the release of p-nitrophenol, which can be measured spectrophotometrically. This makes it a valuable tool in enzyme kinetics studies and in the characterization of enzyme inhibitors. Additionally, it is employed in the development of diagnostic assays for enzyme-related diseases and in the screening of potential therapeutic agents targeting glycosidase enzymes. Its specificity and sensitivity make it a preferred choice in both academic and industrial research settings.