Ethyl α-D-Thioglucopyranoside is primarily used in biochemical research as a substrate or inhibitor in studies involving glycosidases and glycosyltransferases. These enzymes play crucial roles in carbohydrate metabolism, and this compound helps in understanding their mechanisms and interactions. It is also employed in the synthesis of glycosides and other carbohydrate derivatives, aiding in the development of pharmaceuticals and bioactive molecules. Additionally, it serves as a valuable tool in glycobiology for probing cellular processes related to glycosylation, which is essential for protein function and cell signaling.