This compound is widely used in biochemical research as a substrate for studying the activity of glycosidases, particularly β-galactosidases. Its structure mimics natural oligosaccharides, making it valuable for investigating enzyme kinetics and specificity. It is also employed in the development of diagnostic assays to measure enzyme activity in biological samples. Additionally, it serves as a tool in glycobiology research to understand carbohydrate-protein interactions and glycosylation processes. Its synthetic nature allows for controlled experiments, aiding in the exploration of enzymatic pathways and the development of potential therapeutic agents targeting glycosidase-related diseases.