β-glycosidase-IN-1 is primarily used in biochemical research as an inhibitor of β-glycosidase enzymes. These enzymes are involved in the breakdown of glycosidic bonds in complex carbohydrates. By inhibiting β-glycosidase, this compound helps scientists study the role of these enzymes in various biological processes, such as carbohydrate metabolism and cellular signaling. It is also used in the development of therapeutic agents targeting diseases where glycosidase activity is dysregulated, such as lysosomal storage disorders and certain types of cancer. Additionally, β-glycosidase-IN-1 is employed in agricultural research to investigate plant carbohydrate metabolism and improve crop resistance to pathogens.