Widely used in biochemical and enzymatic assays, this compound serves as a fluorogenic substrate for studying protease activity, particularly gamma-glutamyl transpeptidase and other glutamyl-specific enzymes. Upon enzymatic cleavage, it releases a highly fluorescent product—7-amino-4-methylcoumarin—enabling sensitive detection and quantification of enzyme activity in real time. It is commonly applied in high-throughput screening, cellular imaging, and diagnostic assays where precise monitoring of enzymatic kinetics is required. Its high specificity for target enzymes makes it valuable in research involving cancer biomarkers, liver function, and metabolic disorders.