BPTES is primarily used in research settings as a potent and selective inhibitor of glutaminase, an enzyme involved in the metabolism of glutamine. Its application is significant in studying cancer biology, as many cancer cells rely on glutaminolysis for energy and growth. By inhibiting glutaminase, BPTES helps researchers understand the role of glutamine metabolism in tumor progression and survival. This has potential implications for developing targeted cancer therapies that disrupt the metabolic pathways essential for cancer cell proliferation. Additionally, BPTES is utilized in neuroscience research to explore the relationship between glutaminase activity and neurological disorders, such as epilepsy and neurodegenerative diseases. Its ability to modulate glutamine metabolism makes it a valuable tool for investigating cellular energy dynamics and disease mechanisms.