ML324 is primarily used in biochemical and pharmacological research as a selective inhibitor of the enzyme glutaminase C (GAC), which plays a key role in cancer cell metabolism. By targeting GAC, ML324 disrupts glutamine metabolism, a process many tumor cells rely on for energy production and biosynthesis, making it a valuable tool in studies of cancer cell energetics and potential therapeutic strategies. It has been shown to reduce proliferation and induce cell death in various cancer cell lines, particularly in solid tumors and leukemia models. Researchers also use ML324 to investigate metabolic reprogramming in hypoxic conditions, where cancer cells shift their metabolic pathways to support survival and growth. Due to its specificity, ML324 helps elucidate the role of glutaminase in cellular signaling, redox balance, and mitochondrial function, supporting the development of metabolism-targeted anticancer drugs.