Glutaminase-IN-1 is primarily used in research settings to study the role of glutaminase enzymes in cellular metabolism. It is a potent inhibitor that helps in understanding the metabolic pathways involved in cancer cell proliferation, as cancer cells often rely on glutaminolysis for energy and growth. By inhibiting glutaminase activity, researchers can explore potential therapeutic strategies for targeting cancer metabolism. Additionally, it is used in biochemical assays to investigate the effects of glutaminase inhibition on amino acid metabolism and its implications in various diseases, including neurodegenerative disorders and immune responses. Its application extends to drug discovery, where it serves as a tool compound for developing novel inhibitors targeting glutaminase-dependent processes.