Glutaminase C-IN-1 is primarily utilized in research focused on cancer therapy, particularly in targeting cancer cell metabolism. It acts as an inhibitor of glutaminase C, an enzyme that plays a critical role in the metabolism of glutamine, a key nutrient for many cancer cells. By inhibiting this enzyme, it disrupts the ability of cancer cells to proliferate and survive, especially in environments where glucose is limited. This makes it a valuable tool in studies aiming to develop metabolic inhibitors as a strategy to starve cancer cells of essential resources. Additionally, it is used in biochemical assays to better understand the role of glutaminase in cellular processes and disease progression. Its application is significant in the field of oncology and metabolic research, offering potential pathways for therapeutic intervention.