DL-AP4 is primarily used in neuroscience research as a selective agonist for group III metabotropic glutamate receptors (mGluRs). It helps in studying the role of these receptors in synaptic transmission and plasticity, particularly in the central nervous system. Researchers utilize it to explore its potential in modulating neurotransmitter release and its effects on neuronal excitability. Additionally, DL-AP4 is employed in experimental models to investigate its neuroprotective properties and its implications in neurological disorders such as epilepsy, Parkinson's disease, and chronic pain. Its ability to inhibit synaptic activity makes it a valuable tool for understanding glutamate receptor function and developing therapeutic strategies.