TSA Agar is widely used in microbiology for the cultivation and maintenance of a broad range of non-fastidious and fastidious microorganisms. It serves as a general-purpose growth medium suitable for the isolation and routine subculturing of bacteria from clinical, environmental, and food samples. Due to its rich nutrient composition, it supports the growth of pathogens such as Staphylococcus, Streptococcus, and Pseudomonas, making it essential in diagnostic laboratories. TSA Agar is also commonly used for antimicrobial susceptibility testing, colony counting, and quality control of sterile products. When supplemented with blood or other additives, it can be adapted for more specialized applications, such as detecting hemolytic activity. Its reliability and consistency make it a standard medium in both research and industrial settings.