SOB Medium is widely used in molecular biology for the growth of bacterial cultures, especially in transformation experiments. It provides a rich nutrient environment that supports high-efficiency recovery of competent cells after heat shock or electroporation. The medium is particularly favored in cloning workflows where maximum transformation efficiency is required. Due to its optimized composition, SOB allows rapid cell growth and protein expression, making it suitable for plasmid preparation and recombinant protein production. It is often used in place of LB medium when higher transformation yields are needed.