Peptone Salt Solution is widely used in microbiology and biotechnology as a nutrient medium for the cultivation of microorganisms. It supports the growth of various bacteria and fungi by providing essential nitrogen sources, amino acids, and peptides derived from protein digestion. The solution is commonly employed in laboratories for bacterial enrichment, culture maintenance, and propagation prior to further analysis or fermentation processes.

It is also utilized in industrial applications such as seed culture preparation in fermentation tanks, where consistent and rapid microbial growth is required. Due to its simple composition and effectiveness, it serves as a base for more complex media or as a diluent in microbiological testing.

In pharmaceutical and food industries, Peptone Salt Solution plays a role in quality control testing to detect microbial contamination. Its reliability in supporting microbial growth makes it suitable for sterility tests and validation of disinfection procedures.