(−)-Bicuculline methochloride is primarily used in neuroscience research as a potent and selective antagonist of GABA_A receptors. It blocks the inhibitory effects of GABA, the main inhibitory neurotransmitter in the central nervous system, allowing researchers to study the role of GABAergic signaling in various neural processes. This compound is particularly valuable in electrophysiological studies to investigate synaptic transmission, neuronal excitability, and the mechanisms underlying epilepsy and other neurological disorders. Additionally, it is employed in experimental models to explore the effects of reduced GABAergic inhibition on brain function and behavior. Its application extends to pharmacological studies aimed at understanding the therapeutic potential of modulating GABA_A receptors.