RS-25344 hydrochloride is primarily utilized in research settings as a selective agonist for the 5-HT2B receptor. This receptor subtype is of significant interest in studies related to serotonin signaling and its physiological effects. Researchers employ RS-25344 hydrochloride to investigate the role of 5-HT2B receptors in various biological processes, including cardiovascular function, gastrointestinal motility, and central nervous system activity. Its application is crucial in understanding receptor-specific mechanisms and developing targeted therapeutic agents for conditions influenced by serotonin pathways. Additionally, it serves as a valuable tool in pharmacological studies to explore receptor-ligand interactions and their downstream effects.