Used as a fluorescent probe in biological imaging due to its ability to emit light upon binding to specific cellular components. It is particularly effective in detecting changes in membrane potential and monitoring cellular processes in real time. Also employed in the development of chemosensors for detecting metal ions and anions in environmental and biological samples. Its strong electron-withdrawing nitro group enhances sensitivity in sensing applications. Additionally, it serves as an intermediate in synthesizing pharmaceuticals and agrochemicals with bioactive properties.