Used primarily as an intermediate in the synthesis of pteridine-based pharmaceuticals and bioactive compounds. It plays a key role in the development of antifolate agents and kinase inhibitors due to its ability to mimic natural pterin structures. Commonly employed in medicinal chemistry for constructing enzyme inhibitors targeting metabolic pathways in cancer and infectious diseases. Also utilized in the preparation of fluorescent probes and sensors owing to the inherent photophysical properties of the pteridine scaffold. Its reactivity allows for selective derivatization, making it valuable in building complex heterocyclic systems for drug discovery and biochemical research.