Biotin-PEG3-acetic acid is widely used in bioconjugation and biochemical research due to its ability to link biotin with other molecules. Its PEG spacer enhances solubility and reduces steric hindrance, making it ideal for attaching biotin to proteins, peptides, or other biomolecules. This compound is particularly valuable in the development of biotinylated probes for applications such as immunoassays, fluorescence microscopy, and affinity chromatography. It also plays a key role in studying protein interactions and cellular processes by enabling the precise labeling and detection of target molecules. Additionally, it is utilized in drug delivery systems to improve the targeting and stability of therapeutic agents.