This compound is widely used in bioconjugation and biochemical research due to its ability to link biomolecules with high specificity. It is particularly valuable in the preparation of biotinylated probes for applications such as immunoassays, protein labeling, and cellular imaging. The PEG4 spacer provides enhanced solubility and flexibility, reducing steric hindrance and improving binding efficiency in target recognition. Additionally, it is employed in the development of drug delivery systems, where it facilitates the attachment of therapeutic agents to targeting moieties, enhancing precision and efficacy. Its role in studying protein-protein interactions and molecular recognition processes makes it a crucial tool in biomedical research.